STAGE 1/2018

Project Activities

• Activity 1.1.

  • Selection and characterization of marine sources for the isolation of bioactive compounds

• Indicators of achievement:

• 1 study on potential marine sources for the extraction of protein and polysaccharide compounds

• 2 marine organisms selected by species - Rapana venosa and Cladophora vagabunda

• Activity 1.2.

  • Establishing technologies for obtaining protein and polysaccharide extracts from selected sources

• Indicators of achievement: 5 Technological parameters for obtaining protein and polysaccharide extracts from marine organisms (pretreatments, extraction and purification) such as: type and concentration of extraction agent, tissue / solvent ratio, tissue / enzyme ratio, type and concentration of the substance used for purification, temperatures and working times.

• Activity 1.3.

  • Elaboration of experimental methods for the characterization of protein and polysaccharide extracts from the selected sources

• Indicators of achievement:

  • 4 work protocols:

    • a) 1 protocol for the quantitative determination of collagen;

    • b) 1 quantitative determination protocol of GAG (acid polysaccharides and uronic acids);

    • c) 1 quantitative in vitro biocompatibility assessment protocol;

    • d) 1 protocol for the determination of antimicrobial activity.

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STAGE II

1st COMPONENT PROJECT 

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Activity 2.7. Activity name: Obtaining protein and polysaccharide compounds from marine sources through advanced technologies

Indicators of achievement: 2 methods of analysis of marine species: analysis of heavy metals and analysis of pesticides; 4 types of purified bioactive compounds: COL, GEL, GAG, Pz of green algae

Activity 2.8. Activity name: Physico-chemical and biochemical analysis of protein and polysaccharide compounds from marine sources

Performance indicators: 3 optimized analysis methods:

- the electrophoresis method specific for highlighting GAG samples extracted from Rapana venosa

- FTIR spectroscopy method optimized for the evaluation of collagen and gelatin samples

- DSC method optimized for determining the distortion / melting temperature of collagen and gelatin samples

Activity 2.9. Activity Name: Evaluation of biocompatibility of protein and polysaccharide compounds in in vitro experimental models using cell cultures

Indicators of achievement: - 3 bulletins for in vitro biological analysis of the biocompatibility of GAG, COL and GEL samples according to the European standard SR EN ISO 10993-5 / 2009

Activity 2.10. Activity Name: Testing the antimicrobial activity of protein and polysaccharide compounds on different microbial strains

Indicators of achievement: 2 compounds with selected antimicrobial activity: hydroethanol extract of 70% of the green algae species Cladophora vagabunda of 10% concentration, obtained by refluxing for 2 hours and respectively the hydroethanol extract of 96% of the green algae species Cladophora vagabunda of 10% concentration, obtained by cold maceration

Activity 2.11. Activity name: Collection and drying of biological waste resulting from intermediate technological processes for conversion to obtain a biostimulator-regenerator for wine soils

Indicators of achievement: 1 protocol for biological waste processing

Activity 2.12 and Activity 2.14. Activity name: Dissemination by national and international communication and publication of results

Indicators of achievement: 4 articles published in ISI journals; 1 accepted for publication in ISI magazine, 2 articles published in BDI magazine, 24 papers communicated at international conferences (oral communications and posters), 1 workshop

Activity 2.13. Activity Name: Innovation activity and patent application filing

Indicators of achievement:

- 1 patent application filed with OSIM

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3 rd STAGE/ 2020

COMPONENT PROJECT NO. 1

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• Activity 3.1.: Development of dermato-cosmetic products and their conditioning in different forms - Part 1

Achievement indicators:

• 2 biotechnologies

• 3 variants of conditioning dermato-cosmetic products

• Activity 3.2.: Physico-chemical and microbiological evaluation of dermato-cosmetic products - Part 1

Achievement indicators:

• 1 method of antioxidant activity analysis

• 1 bulletin for the analysis of physico-chemical parameters

• 1 analysis bulletin for antimicrobial activity 

• Activity 3.3.: Demonstration of the therapeutic effect of the products obtained on optimal experimental models in cell cultures specific to dermal tissue - Part 1

Achievement indicators:

• 2 optimized methods for biocompatibility analysis

• 1 in vitro experimental model 

• Activity. 3.4.: Selection of optimal variants and development of prototype products with superior dermato-cosmetic efficiency - Part 1

Achievement indicators:

• 2 dermato-cosmetic products

• Activity 3.5.: Processing of biological waste resulting from intermediate technological processes for conversion to obtain a biostimulator-regenerator for viticultural soils - Part 1

Achievement indicators:

• 1 biostimulator-regenerator - preliminary version 

• Activity 3.11.: Dissemination of results - Part 1

Achievement indicators:

• 1 paper communicated at a scientific event

• 1 article published in the ISI / BDI magazine

• Activity 3.12.: Innovation activity and patent application - Part 1

Achievement indicators:

• 1 patent application

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 Stage no. III / 2020 - Dissemination of results

 

Component project no. 1

PATENT OF INVENTION 

"Biostimulating-regenerating composition based on residual biomass with fertilization potential", National Patent Application, OSIM, 2020

 

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