SUMMARY OF 2nd STAGE / 2019

​

    In order to achieve the objectives proposed in this stage, within the project component 2, several polyphenolic extracts from different tescovines were prepared which were characterized not only by spectrophotometric methods, but also by HPLC, apparatus purchased from the project. Regarding the chemical profile, in all the extracts prepared both by conventional extraction and in the microwave field were identified: gallic acid, vanilic, syringic, protocatehic, (-) epicatechin and trans-resveratrol, while chloride of pelargonidine and mericetin were found only in Cabernet Sauvignon (CS) extract. The polyphenolic extracts were encapsulated in mesoporous silica matrices of pure MCM-41 type and functionalized with both heteroatoms (Zn or Mg) and organic groups. The encapsulated extracts showed superior antioxidant activities compared to the free ones due to the better stability over time of the encapsulated extract. Cell viability assays performed on human HaCaT keratinocytes demonstrated very good biocompatibility of CS extract that stimulates cell proliferation, both free and encapsulated in the Zn-MCM-41 matrix, which exhibited good cytocompatibility, being a potential candidate for cosmetic formulations. or nutraceuticals. Also at this stage, colloidal-silica colloidal / TiO2-type composites were obtained and characterized. Also, the optimal technological parameters for the extraction of polysaccharides from the green algae Ulva-lettuce were determined, which had a lower content of heavy metals than other types of green algae. The polysaccharide extract exhibited good biocompatibility, being non-cytotoxic up to a concentration of 3 mg / mL at 24 h, and up to 2 mg / mL at 48 h. Another goal of this step was to obtain preliminary pharmaceutical formulations. Thus, 4 variants of hydrogels were prepared in which both polyphenolic and green algae extracts were included. For a higher recovery of both the algal biomass and the residual biomass from the vinification, the possibility of obtaining biostimulator-regenerative variants for vineyards by mixing the residual algal marine biomass, the shells and the tescovina was studied, 7 mixtures were prepared with different compositions that have been tested on four types of cultures.

    Within the component project 1 two methods of analysis of the marine species Rapana venosa and Cladophora vagabunda and the analysis of heavy metals and pesticides were established. 4 types of purified bioactive compounds were obtained: collagen, gelatin, glycosaminoglycans from rapeseed and polysaccharides from algae by optimized obtaining technologies. There were 3 optimized analysis methods: the electrophoresis method for highlighting the GAG ​​samples extracted from Rapana venosa, the optimized FTIR spectroscopy method for evaluating collagen and gelatin samples, the optimized DSC method for determining the temperature of denaturing / melting of collagen samples. and gelatin and the biocompatibility of GAG, COL and GEL samples was tested in vitro in the INCDSB cell culture laboratory, according to the European standard SR EN ISO 10993-5 / 2009. Antimicrobial activity was also evaluated for the extracts and 2 compounds with antimicrobial activity were selected, respectively: 70% hydroethanol extract of the green algae species Cladophora vagabunda of 10% concentration, obtained by reflux and hydroethanol extract of the same species, obtained by cold maceration. The obtained results determined the fulfillment of all the objectives, these being promising in the perspective of the elaboration of new pharmaceutical and dermato-cosmetic preparations that include extracts that have the expected therapeutic effects. Therefore, by carrying out the activities from this stage, all the achievement indicators were met and exceeded, as well as the objectives proposed, and the value of the obtained results is supported by the dissemination activity, being presented in 24 conferences and congresses as well as by publishing 5 articles. ISI (3 in Q1 area journals), 2 BDI indexed articles and 1 patent application (OSIM). Also, the infrastructure of the partners continued to be improved and the service offer diversified. All 6 new young researchers, with very good professional training, were actively involved in carrying out the activities, including disseminating the results of the complex project, a paper presented by a young researcher was awarded for the best poster, at the international conference.

​

​

​