ABSTRACT

The project entitled “INNOVATIVE NANOSTRUCTURED NUTRACEUTICALS WITH SYNERGISTIC BIOACTIVITIES FOR HEPATO-DIGESTIVE PROTECTION (NUTRASINPRO)” falls in the priority research domain of the present competition call, Bioeconomy, Subdomain Biotechnology. The general objective is obtaining of controlled nanostructure delivery systems based on natural raw materials mixtures as fluid fruits extracts of bitter cherry (Prunus avium var. sylvestris L.) and blue honeysuckle (Lonicera caerulea L.), grape pomace (Vitis vinifera L.) as by-product of grape winemaking encapsulated into a hybrid silica–barley germs mass (Hordeum vulgare) as prebiotics, nanomatrix enriched with Lactobacillus sp. strains as probiotic. The main objectives of the project are design of new nutraceuticals formula obtained from high functional food-grade bioactive agents (anthocyanins, carotenoids, polyphenols, flavonols, flavones, vitamins, lipids, oligominerals) present in vegetal extracts encapsulated within a hybrid silica matrix-barley germs biomass nanostructure in different ratio; obtaining an innovative series of nutraceuticals  delivery nanosystems exhibiting synbiotic action of prebiotics–probiotics constituents with synergistic therapeutically action; controlled delivery systems by nanoscale targeted level, with synergistic bioactivities, antimicrobial, antioxidant, anti-inflammatory and hepato-digestive protection and development of new technologies to obtain nutraceuticals nano-formulations conditioned as orodispersible tablets, controlled release tablets and lyophilized powder for oral use, protected by property rights. The envisaged end products: 1. Patenting the optimal variant nanostructured nutraceutical formulas of the new nanostructured target delivery system and issued in improving oral bioavailability for all ages group; 2. Technological process development for widely implementation of nanostructured nutraceutical formulations, used without risk on human, animal or environment health.

 

PROJECT OBJECTIVE

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Obtaining of new nanostructured nutraceutics and development an innovative soft-technology for the production of safe, efficient, innovative nanostructured target delivery system based on phyto-complex with bitter cherry, blue honeysuckle fruits and grape pomace as fluid extracts, nanoencapsulated in hybrid mesoporous silica–prebiotic barley germs mass nanomatrix enriched with probiotic Lactobacillus sp. strains.

 

 ESTIMATED RESULTS

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In correlation with the objectives proposed in the project, will be made: project website, methodologies and experimental models developed for fluid extracts of bitter cherry fruits, blue honeysuckle fruits and red grape pomace, biotechnological model for the synergistic combination of mixture from fluid extracts from bitter cherry fruits, blue honeysuckle fruits and red grape pomace with antimicrobial and antioxidant activities, technological model of mesoporous hybrid nanomatrix silica-prebiotic barley germs loaded with fluid fruit extracts associated or not with a suitable probiotic strains Lactobacillus sp., functional models of solid nutraceutical formulations with controlled release based on mesoporous hybrid nanomatrix silica-prebiotic barley germs loaded with extracts of bitter cherry fruits, blue honeysuckle fruits and red grape pomace with or without probiotic strains Lactobacillus sp., nanostructured nutraceuticals conditioned as orodispersible tablets, controlled release tablets and lyophilized powders for oral use, tests to verify the synergistic effects of hepato-digestive protection, scientific articles published in WoS journals, participation with communications at international conferences, patent applications at OSIM, agreement collaboration between partners.

 

Contract start date: 07/01/2022

Date of conclusion of the contract: 30/06/2024

Project duration: 24 months

Number of institutions involved: 4

- Coordinating institution: Ovidius University of Constanta

- Partner P1: National Research-Development Institute for Biological Sciences

- Partner P2: Polytechnica University of Bucharest

- Partner P3: Research-Development Station for Viticulture and Winemaking Murfatlar

Total value of the Contract: 598,795.00 Lei

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PRESENTATION OF THE RESULTS OBTAINED IN THE

PROJECT NUTRASINPRO

 

The NUTRASINPRO project has led to new patent-pending solid nutraceutical formulations in the form of siloxane nanomatrixes as controlled delivery systems of phytocomplex-prebiotic-probiotic mixtures with significant potential to regulate digestion, liver activity and intestinal transit. The patent-pending processes for obtaining nanostructured nutraceutical preparations at the laboratory level enhance the therapeutic effect and synergistic bioactivity of fluid extracts obtained from selected mixtures of bitter cherry (Prunus avium var. sylvestris L.), blue honeysuckle (Lonicera caerulea L. ), Mamaia variety red grape pomace (Vitis vinifera L.) by-product of the vinification process, as well as a fluid extract of green barley (Hordeum vulgare L.) germ with prebiotic effect, associated with a mixture of Lactobacillus sp. strains as probiotic, encapsulated in a nanostructured mesoporous silica nanomatrix.

The results of the project demonstrated that, the new solid nanostructured nutraceutical formulations of orodispersible tablets, extended-release tablets and orally dispersible powders of natural origin are non-cytotoxic and safe for consumption by all ages, are an effective alternative to marketed synthetic products, are necessary for the field of human health and bioeconomy through the development of environmentally friendly, eco-friendly and biotechnologies.

The nutraceutical preparations resulting from the project present a number of therapeutic advantages, such as increased antioxidant activity, high hepato-digestive protection potential, probiotic and prebiotic effect for the development and maintenance of gastrointestinal tract health.

 

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Summary technological scheme for the obtaining of nanostructured nutraceuticals with synergistic hepato-digestive protection bioactivities for oral use according to the

NUTRASINPRO project

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